Journal Details
Palynology
Aims & Scope
©2011 Thomson Reuters, 2010 Journal Citation Reports®
2. infiltrated with 2,2'-thiodiethanol (TDE), a mounting medium for high-resolution microscopy
3. A 3-D image stack was acquired using an Olympus FV1000 laser scanning confocal microscope with a 100x/1.4 oil immersion objective (Excitation 488 nm, emission 500-600nm)
4. Imported the entire confocal stack into Osiri X 3D medical imaging software running on an iMac with 4GB of RAM.
5. Selected every fifth slice in the stack for rendering, in order to fit the stacks into the 4GB memory limit addressable by a 32-bit operating system
6. Perform 3D rendering using a color map optimized for bones and soft tissue
7. Adjusted the color map and transparency, in order to show both the exterior surface textures and the interior wall structures
Work for this image was performed by Vaughn M. Bryant, Stanislav Vitha, and Amen Zwa
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